Two CLICK-functionalized adenosine analogs - N 6-Propargyl-Adenosine (N 6pA) and 2-Ethynyl-Adenosine (2-EA) have been reported as suitable substrates for poly(A) tail synthesis (polyadenylation) monitoring in mammalian cells. induced by changes in signaling pathways. Traditional methods for the analysis of poly(A) tails do not allow the discrimination between preexisting and newly polyadenylated mRNA transcripts e.g. by influencing mRNA stability, nuclear export and translation efficiency. This modification plays a critical role in mRNA metabolism and thus gene regulation e.g. Poly(A) tails, a long chain of adenosine nucleotides of variable length, are posttranscriptionally added to the 3'-end of eukaryotic mRNAs by poly(A) polymerase (polyadenylation). RNA Labeling Selector (Kits & Nucleotides)ĬLICK-functionalized adenosine (triphosphate): Analysis of newly synthesized poly(A) tails in vivo and in vitro.DNA Labeling Selector (Kits & Nucleotides).Privacy Policy for Jena Bioscience's Facebook Page.Poly (A) carrier RNA-based RNA purification.3'-End RNA Labeling (T4 RNA Ligase 1-based).Random RNA Labeling ( in vitro Transcription-based).Click Chemistry-based RNA/cRNA Labeling.Click Chemistry-based DNA/cDNA Labeling.Quantifoil® Holey Carbon Films with 2 nm Continuous Carbon.Mercurated and Selenium-containing Nucleotides.JBS Tungsten Cluster Derivatization Kits.Cryo and Room Temperature Crystallography.in Posttranslational Modification Analysis Immobilized Nucleotides for Affinity Chromatography.Stable Isotope labeled Nucleosides & Nucleotides.on Proteins/Enzymes - Sulfonyl Fluoride Probes Cancer and Proliferation Marker Nucleosides.
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